"Yesterday, we learned that Aeroflot, just like last week, canceled this week's flight and next week's flight for some reason known to them," Tadas Vasiliauskas told BNS. "They gave us no further explanation."
"The plane will not come from Moscow, meaning that the flight from Vilnius will not take off either," he added.
This follows the cancelation of Aeroflot's weekly flight to Vilnius last Friday.
Last week, Lithuania banned any flights to or from its airports via Belarusian airspace and closed its airspace to Belarusian airlines.
The move came in response to Belarus' forcing down of a Ryanair flight from Athens to Vilnius flight.
In the wake of the incident, EU leaders agreed to ban Belarusian airlines from the bloc's airspace and called on EU-based air carriers to avoid Belarus' airspace.